Concentration, reconstitution and dilution for lyophilised research peptides — aimed at a clean, easy-to-measure draw. In-vitro research use only.
Choose your syringe, vial and sample size, then press Calculate for recommended mixing volumes.
You’ll need bacteriostatic water to mix your peptide. Add it here, or browse research peptides, all dispatched tracked from inside the EU.
This free tool is a bench aid for laboratory research: it turns three numbers into a clean, measurable draw. Everything below describes reconstitution arithmetic for research reagents only. It is not dosing guidance, and the figures are measurement aids, not instructions for use in people or animals.
Reconstitution is simple arithmetic once you have three figures: the peptide mass in the vial (milligrams), the volume of bacteriostatic water you add (millilitres), and the sample size you want to draw. Dividing the vial mass by the water volume gives the concentration in mg per mL. A 10 mg vial made up with 2 mL of bacteriostatic water sits at 5 mg/mL. To find how much to draw for a target sample, divide the sample size by that concentration. The calculator above does this for you and also converts the answer into syringe units, so you do not need a separate conversion chart.
The amount of water you add is a research decision, not a fixed rule. Adding more water lowers the concentration, which spreads the same sample across a larger, easier-to-read volume on the syringe. Adding less water raises the concentration and shrinks the draw, which can be hard to measure accurately on a fine scale. A practical aim is a mixing volume that lands your target sample around the middle of the barrel rather than at the very first mark. Bacteriostatic water (sterile water with 0.9% benzyl alcohol) is the usual diluent because the preservative lets a multi-use vial be entered more than once. Add it slowly down the inside wall of the vial and swirl gently rather than shaking.
Research insulin-style syringes are graduated in units, not millilitres. On a U-100 syringe, 1 mL is divided into 100 units, so one unit equals 0.01 mL. If the calculator tells you to draw 0.05 mL, that is 5 units on the barrel; 0.2 mL is 20 units. Working in whole units is usually easier at the bench because the marks are clearly printed. The tool reports both the millilitre volume and the unit count for every result, alongside the concentration, so you can cross-check the figure against the syringe in front of you.
This free peptide calculator helps researchers work out reconstitution, concentration and sample size in the lab — including for Retatrutide, TB-500, GHK-Cu, CJC-1295 and other lyophilised peptides used in in vitro studies. Enter your vial amount in mg, your mixing water in mL and a required sample size in mcg or mg. The tool returns the volume to measure and graduated units for common U-100 laboratory syringes.
Also referred to as a peptide concentration calculator, peptide reconstitution calculator, peptide dilution calculator, or research peptide calculator — it converts mg↔mL and mcg↔IU and suggests easy measurement points for typical research vials.
Scenario: 10 mg peptide, want 250 mcg sample
Recommendation: Add 2 mL bacteriostatic water
Result: Measure 0.05 mL (5 IU) for ~250 mcg sample
Scenario: 5 mg peptide, want 500 mcg sample
Recommendation: Add 1 mL bacteriostatic water
Result: Measure 0.1 mL (10 IU) for ~500 mcg sample
For in vitro research use only. Illustrative measurement scenarios — not human or veterinary guidance.
Vial: 10 mg lyophilised Retatrutide
Reconstitution: 2 mL bacteriostatic water → 5 mg/mL
500 mcg research sample: measure 0.1 mL (10 IU)
Vial: 5 mg lyophilised TB-500
Reconstitution: 2 mL bacteriostatic water → 2.5 mg/mL
500 mcg research sample: measure 0.2 mL (20 IU)
Vial: 50 mg lyophilised GHK-Cu
Reconstitution: 5 mL bacteriostatic water → 10 mg/mL
1 mg research sample: measure 0.1 mL (10 IU)
Vial: 2 mg lyophilised CJC-1295
Reconstitution: 2 mL bacteriostatic water → 1 mg/mL
100 mcg research sample: measure 0.1 mL (10 IU)
How do I reconstitute lyophilised peptides?
Add bacteriostatic water slowly to your peptide vial. Use the calculator to determine the volume needed for your intended concentration. For in vitro research only.
What syringe size is shown?
U-100 graduated syringes, selectable as 0.3 mL, 0.5 mL, or 1 mL.
How do I convert mg to mcg?
1 mg equals 1000 mcg. The calculator keeps both in sync.
What's the difference between mL and IU?
mL is volume. IU are unit markings on U-100 graduated syringes where 1 mL equals 100 IU.
Does this calculator support Retatrutide, TB-500, GHK-Cu and other research peptides?
Yes — it works with any lyophilised peptide. Select the vial size in mg, enter the bacteriostatic water volume and your required sample size. Output is for in vitro laboratory research only.
What's a research peptide concentration calculator?
A research peptide concentration calculator converts your vial weight (mg) and bacteriostatic water volume (mL) into a concentration (mg/mL), then helps you measure a target sample size on a U-100 graduated syringe. Also called a peptide reconstitution calculator, dilution calculator, or research peptide calculator.
For research purposes only. Not for human consumption. This tool is a reconstitution aid for laboratory research and does not constitute dosing advice.
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